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a OVCAR8 cells had been incubated with 10??M EdU, in advance of becoming handled with automobile, one hundred?nM CX-5461, one hundred?nM BMN-673 or the combination of both equally for twenty-four?h. Co-IF for ?H2AX and RAD51 was done. Cells were being incubated for thirty?minutes at space temperature with Click on-IT response, washed with PBS and t

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